Goat anti-PPP2R5A Antibody

Item#:

dAP-0324

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PPP2R5A Antibody
Principle Name	PPP2R5A
Official Symbol	PPP2R5A
All Name and Symbols	PPP2R5A; protein phosphatase 2, regulatory subunit B (B56), alpha isoform; MGC131915 ; PP2A, B subunit, B' alpha isoform; PP2A, B subunit, B56 alpha isoform; PP2A, B subunit, PR61 alpha isoform; PP2A, B subunit, R5 alpha isoform; serine/threonine protein
Accession Number(s)	NP_006234.1
Peptide Sequence	AYNMHSILSNTSAE, is from C Terminus
Human Gene ID(s)	5525
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 55kDa band observed in human heart and skeletal muscle lysate (calculated MW of 56.2kDa according to NP_006234.1). Recommended for use at 0.5-2µg/ml.
Immunohistochemistry	In paraffin embedded Human Heart shows textured cytoplasm staining in cardiomyocytes. Recommended concentration, 5-10µg/ml.
Reference(s)	McCright B, Virshup DM. Identification of a new family of protein phosphatase 2A regulatory subunits. J Biol Chem 1995 Nov 3;270(44)