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Goat anti-HMHA1 / HA-1 Antibody
Item#:
dAP-0317
Size Price Quantity
50.0 ug $310.00
Goat anti-HMHA1 / HA-1 Antibody
Principle Name HMHA1 / HA-1
Official Symbol HMHA1
All Name and Symbols HA-1; minor histocompatibility antigen HA-1; HLA-HA1; KIAA0223; HMHA1; histocompatibility (minor) HA-1
Accession Number(s) NP_036424.2
Peptide Sequence TLGSCRERQPEFV, is from C Terminus
Human Gene ID(s) 23526
Non-Human GeneID(s)
Important Comments To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and won't recognise the peptide found eluted from HLA-A*0201 molecules by den Haan et al (Science. 1998 Feb 13;279(5353)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 65-70kDa band in Jurkat lysate at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 131kDda according to NP_
Reference(s) den Haan JM, Meadows LM, Wang W, Pool J, Blokland E, Bishop TL, Reinhardus C, Shabanowitz J, Offringa R, Hunt DF, Engelhard VH, Goulmy E The minor histocompatibility antigen HA-1

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