Goat anti-TCP1 Antibody |
| Principle Name | TCP1 |
| Official Symbol | TCP1 |
| All Name and Symbols | TCP1; t-complex 1; CCT-alpha; CCT1; CCTa; D6S230E; TCP-1-alpha; T-complex protein 1; T-complex protein 1, alpha subunit; tailless complex polypeptide 1 |
| Accession Number(s) | NP_110379.2; NP_001008897.1 |
| Peptide Sequence | SYEDAVHSGALND, is from C Terminus |
| Human Gene ID(s) | 6950 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise both reported isoforms. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 60kDa band observed in Human Lung and Human Testis lysates (predicted MW of 60kDa according to NP_110379.2). Recommended for use at 0.5-2µg/ml. Primary incubated for 1 hour. |
| Immunohistochemistry | In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration |
| Reference(s) | Yaffe MB, Farr GW, Miklos D, Horwich AL, Sternlicht ML, Sternlicht H. TCP1 complex is a molecular chaperone in tubulin biogenesis. Nature. 1992 Jul 16;358(6383) |
