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Goat anti-P114-RHO-GEF / ARHGEF18 Antibody
Item#:
dAP-0311
Size Price Quantity
50.0 ug $310.00
Goat anti-P114-RHO-GEF / ARHGEF18 Antibody
Principle Name P114-RHO-GEF / ARHGEF18
Official Symbol ARHGEF18
All Name and Symbols ARHGEF18; Rho/Rac guanine nucleotide exchange factor (GEF) 18; P114-RHO-GEF; KIAA0521; MGC15913; Rho-specific guanine nucleotide exchange factor p114; P114-RhoGEF; Rho/Rac guanine nucleotide exchange factor 18
Accession Number(s) NP_056133.2; NP_001124427.1
Peptide Sequence SAKEDASKEDVIFF, is from C Terminus
Human Gene ID(s) 23370
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 140+90kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114kDa according to NP_056133.2 and 131kDa accoeding to NP_001124427.1). Recommended concentration
Reference(s) Blomquist A, Schworer G, Schablowski H, Psoma A, Lehnen M, Jakobs KH, Rumenapp U. Identification and characterization of a novel Rho-specific guanine nucleotide exchange factor. Biochem J. 2000 Dec 1;352 Pt 2

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