Goat anti-SH3BP1 Antibody |
| Principle Name | SH3BP1 |
| Official Symbol | SH3BP1 |
| All Name and Symbols | SH3BP1; SH3-domain binding protein 1; SH3 domain binding protein 1; OTTHUMP00000028981 |
| Accession Number(s) | NP_061830.3 |
| Peptide Sequence | PQPRPRSLASETN, is from C Terminus |
| Human Gene ID(s) | 23616 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC, ICC, IF |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 70kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7kDa according to NP_061830.3). Recommended for use at 1-3µg/ml. |
| Immunohistochemistry | In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration |
| Reference(s) | Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill W The DNA sequence of human chromosome 22. Nature. 1999 Dec 2;402(6761) |
