Goat anti-VPS28 Antibody |
| Principle Name | VPS28 |
| Official Symbol | VPS28 |
| All Name and Symbols | VPS28; vacuolar protein sorting 28 (yeast); vacuolar protein sorting 28 homolog (S. cerevisiae); MGC60323; vacuolar protein sorting 28; yeast class E protein Vps28p homolog |
| Accession Number(s) | NP_057292.1 |
| Peptide Sequence | ESAYNAFNRFLHA, is from C Terminus |
| Human Gene ID(s) | 51160 |
| Non-Human GeneID(s) | 66914 (mouse); |
| Important Comments | This antibody is expected to recognize isoform 1 (NP_057292.1) only. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 1000. |
| Western Blot | Approx 24kDa band observed in H460, Human Testis and Brain lysates (predicted MW of 25kDa according to NP_057292.1). Recommended for use at 1-3µg/ml. |
| Immunohistochemistry | In paraffin embedded Human Testis, stains acrosomal head caps in round spermatids. Recommended concentration |
| Reference(s) | Bishop N, Woodman P. TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes. J Biol Chem. 2001 Apr 13;276(15) |
