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Goat anti-4E-T / EIF4ENIF1 Antibody
Item#:
dAP-0291
Size Price Quantity
50.0 ug $310.00
Goat anti-4E-T / EIF4ENIF1 Antibody
Principle Name 4E-T / EIF4ENIF1
Official Symbol EIF4ENIF1
All Name and Symbols EIF4ENIF1; 4E-T; Clast4; FLJ21601; 2610509L04Rik; eukaryotic translation initiation factor 4E nuclear import factor 1; eIF4E-transporter; FLJ26551; OTTHUMP00000063276
Accession Number(s) NP_062817.2; NP_001157974.1
Peptide Sequence AKVISVDELEYRQ, is from C Terminus
Human Gene ID(s) 56478
Non-Human GeneID(s) 74203 (mouse);
Important Comments This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5µg/ml
Immunohistochemistry In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10µg/ml.
Reference(s) Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N. A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E. EMBO J. 2000 Jun 15;19(12)

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