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Goat anti-PAR6alpha / PARD6A Antibody


Item#:
dAP-0290
Size Price Quantity
50.0 ug $310.00
Goat anti-PAR6alpha / PARD6A Antibody
Principle Name PAR6alpha / PARD6A
Official Symbol PARD6A
All Name and Symbols PARD6A; PAR6alpha; PAR-6; TAX40; PAR-6A; TIP-40; par-6 partitioning defective 6 homolog alpha (C.elegans); Tax interaction protein 40; par-6 (partitioning defective 6, C.elegans) homolog alpha; PAR6; PAR6C; Tax-interacting protein 40; par-6 partitioning d
Accession Number(s) NP_058644.1; NP_001032358.1
Peptide Sequence GSRIRGDGSGFSL, is from C Terminus
Human Gene ID(s) 50855
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot Preliminary experiments gave no signal but low background in Human Brain and Pancreas extracts at up to 1µg/ml.
Immunohistochemistry In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration
Reference(s) Johansson A, Driessens M, Aspenstrom P. The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1. J Cell Sci. 2000 Sep;113 ( Pt 18)

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