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Goat anti-PHLDA2 / IPL Antibody
Item#:
dAP-0281
Size Price Quantity
50.0 ug $310.00
Goat anti-PHLDA2 / IPL Antibody
Principle Name PHLDA2 / IPL
Official Symbol PHLDA2
All Name and Symbols PHLDA2; pleckstrin homology-like domain, family A, member 2; TSSC3; IPL; BRW1C; BWR1C; HLDA2; PHLDA2; tumor suppressing subtransferable candidate 3; tumor-supressing STF cDNA 3; imprinted in placenta and liver; p17-Beckwith-Wiedemann region 1C; pleckstrin
Accession Number(s) NP_003302.1
Peptide Sequence EPSRPSPQPKPRTP, is from C Terminus
Human Gene ID(s) 7262
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave no signal but low background in Jurkat and human placenta extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry In paraffin embedded Placenta shows staining of the syncytiotrophoblasts. Recommended concentration, 3-6µg/ml.
Reference(s) Qian N, Frank D, O'Keefe D, Dao D, Zhao L, Yuan L, Wang Q, Keating M, Walsh C, Tycko B. The IPL gene on chromosome 11p15.5 is imprinted in humans and mice and is similar to TDAG51, implicated in Fas expression and apoptosis. Hum Mol Genet. 1997 Nov;6(12)

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