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Goat anti-POLL / POLkappa Antibody
Item#:
dAP-0270
Size Price Quantity
50.0 ug $310.00
Goat anti-POLL / POLkappa Antibody
Principle Name POLL / POLkappa
Official Symbol POLL
All Name and Symbols POLL; BETA-N; POL-KAPPA; polymerase (DNA directed), lambda; polymerase (DNA-directed), lambda; DNA polymerase kappa (DNA polymerase beta-N); RP11-529I10.1; BETAN; FLJ46002; POLKAPPA; DNA polymerase beta-2; DNA polymerase kappa (DNA polymerase beta-N); DNA
Accession Number(s) NP_037406.1
Peptide Sequence LGLPYREPAERDW, is from C Terminus
Human Gene ID(s) 27343
Non-Human GeneID(s) 56626 (mouse);
Important Comments It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 60kDa band observed in cell lysates of A549 and approx 60-70kDa in cell lysates of HepG2 (calculated MW of 63.5kDa according to Human NP_037406.1). Recommended concentration
Reference(s) Garcia-Diaz M, Bebenek K, Sabariegos R, Dominguez O, Rodriguez J, Kirchhoff T, Garcia-Palomero E, Picher AJ, Juarez R, Ruiz JF, Kunkel TA, Blanco L. DNA polymerase lambda, a novel DNA repair enzyme in human cells. J Biol Chem. 2002 Apr 12;277(15)

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