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Goat anti-ANILLIN / Scraps (C Terminus) Antibody
Item#:
dAP-0265
Size Price Quantity
50.0 ug $310.00
Goat anti-ANILLIN / Scraps (C Terminus) Antibody
Principle Name ANILLIN / Scraps (C Terminus)
Official Symbol ANLN
All Name and Symbols ANLN; Scraps; ANILLIN; anillin, actin binding protein (scraps homolog, Drosophila); anillin (Drosophila Scraps homolog), actin binding protein; anillin, actin binding protein; DKFZp779A055; scra
Accession Number(s) NP_061155.2
Peptide Sequence WQPDACYKPIGKP, is from C Terminus
Human Gene ID(s) 54443
Non-Human GeneID(s) 68743 (mouse);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kD
Immunohistochemistry In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules.. Recommended concentration
Reference(s) Oegema K, Savoian MS, Mitchison TJ, Field CM. Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis. J Cell Biol. 2000 Aug 7;150(3)

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