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Goat anti-NET1 / ARHGEF8 (C Terminus) Antibody


Item#:
dAP-0261
Size Price Quantity
50.0 ug $310.00
Goat anti-NET1 / ARHGEF8 (C Terminus) Antibody
Principle Name NET1 / ARHGEF8 (C Terminus)
Official Symbol NET1
All Name and Symbols NET1; ARHGEF8; NET1A; neuroepithelial cell transforming gene 1; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelial cell transforming 1; RP11-116G8.1; p65 Net1 proto-oncogene protein; small GTP
Accession Number(s) NP_001040625.1; NP_005854.2
Peptide Sequence DKALSGGKRKETLV, is from C Terminus
Human Gene ID(s) 10276
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave bands at approx 85kDa in Human Placenta and a very strong band at approx 40-50kDa in Human Skeletal Muscle lysates after 0.1µg/ml staining. Please note that currently we cannot find an explanation in the literat
Immunohistochemistry In paraffin embedded Human Tonsil shows staining of fibrous structures. Recommended concentration
Reference(s) Chan AM, Takai S, Yamada K, Miki T. Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning. Oncogene. 1996 Mar 21;12(6)

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