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Goat anti-NIPP1 / PPP1R8 Antibody
Item#:
dAP-0242
Size Price Quantity
50.0 ug $310.00
Goat anti-NIPP1 / PPP1R8 Antibody
Principle Name NIPP1 / PPP1R8
Official Symbol PPP1R8
All Name and Symbols PPP1R8; NIPP1; protein phosphatase 1, regulatory (inhibitor) subunit 8; ARD1; NIPP-1; PRO2047; ard-1; activator of RNA decay; nuclear subunit of PP-1; homolog of E.coli RNase E; nuclear inhibitor of protein phosphatase-1; protein phosphatase-1, regulatory
Accession Number(s) NP_002704.1; NP_054829.2; NP_612568.1
Peptide Sequence EAWPGKKPTPSLLI, is from C Terminus
Human Gene ID(s) 5511
Non-Human GeneID(s) 100336 (mouse);
Important Comments This antibody is expected to recognise all three human isoforms of this protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 40kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration
Reference(s) Beullens M, Bollen M. The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly. J Biol Chem. 2002 May 31;277(22)

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