Goat anti-NUMBL Antibody |
| Principle Name | NUMBL |
| Official Symbol | NUMBL |
| All Name and Symbols | NUMBL; numb homolog (Drosophila)-like; NBL; CAG3A; CTG3a; NUMBR; NUMB-R; TNRC23; numb (Drosophila) homolog-like; NUMBLIKE; TNRC23; numb homolog-like |
| Accession Number(s) | NP_004747.1 |
| Peptide Sequence | SRSAAASGGPRRPE, is from N Terminus |
| Human Gene ID(s) | 9253 |
| Non-Human GeneID(s) | 18223 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB Trf |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | In transfected HEK293 transiently expressing Human Numbl and DYKDDDDK tagged), a band of approx. 85kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concent |
| Reference(s) | Petersen PH, Zou K, Hwang JK, Jan YN, Zhong W. Progenitor cell maintenance requires numb and numblike during mouse neurogenesis. Nature. 2002 Oct 31;419(6910) |
