Goat anti-RANBPM / RANBP9 Antibody

Item#:

dAP-0230

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-RANBPM / RANBP9 Antibody
Principle Name	RANBPM / RANBP9
Official Symbol	RANBP9
All Name and Symbols	RANBP9; RanBPM; RAN binding protein 9; RANBPM; novel centrosomal protein RanBPM; ran binding protein, centrosomal
Accession Number(s)	NP_005484.2
Peptide Sequence	GSCAFATVEDYLH, is from C Terminus
Human Gene ID(s)	10048
Non-Human GeneID(s)	56705 (mouse);
Important Comments	This product is also expected to recognize the centrosome-associated truncated isoform RANBPM (BAA23216.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC, ICC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments gave no signal but low background in whole cell Hela lysates at up to 1µg/ml.
Immunohistochemistry	In paraffin embedded Human Tonsil, stains nuclei in epithelial cells of tonsillar criptae. Recommended concentration
Reference(s)	Wang D, Li Z, Messing EM, Wu G. Activation of Ras/Erk pathway by a novel MET-interacting protein RanBPM. J Biol Chem. 2002 Sep 27;277(39)