Goat anti-FBXL12 Antibody

Item#:

dAP-0228

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-FBXL12 Antibody
Principle Name	FBXL12
Official Symbol	FBXL12
All Name and Symbols	FBXL12; likely ortholog of mouse f-box and leucine-rich repeat protein 12; FLJ20188; hypothetical protein FLJ20188; F-box and leucine-rich repeat protein 12; Fbl12
Accession Number(s)	NP_060173.1
Peptide Sequence	RACPKESMDWWM, is from C Terminus
Human Gene ID(s)	54850
Non-Human GeneID(s)	30843 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave no signal but low background in Human Brain lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry	In paraffin embedded Human Lung shows staining of ciliated epithelial cells in the bronchioles. Recommended concentration, 4-6µg/ml.
Reference(s)	Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW. A family of mammalian F-box proteins. Curr Biol. 1999 Oct 21;9(20)