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Goat anti-FOXA2 / HNF3B Antibody
Item#:
dAP-0218
Size Price Quantity
50.0 ug $310.00
Goat anti-FOXA2 / HNF3B Antibody
Principle Name FOXA2 / HNF3B
Official Symbol FOXA2
All Name and Symbols FOXA2; HNF3B; forkhead box A2; TCF3B; MGC19807; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta
Accession Number(s) NP_068556.2; NP_710141.1
Peptide Sequence GVYSRPIMNSS, is from C Terminus
Human Gene ID(s) 3170
Non-Human GeneID(s) 15376 (mouse); 25099 (rat);
Important Comments This antibody is expected to recognise both reported isoforms
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Rat
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Approx 60kDa band observed in Human Liver and Rat Lung lysates (calculated MW of 48.3kDa according to Human NP_710141.1 and 48.5kDa according to Rat NP_036875.1). The observed molecular weight corresponds to earlier findings in literature (
Immunohistochemistry In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration
Reference(s) Wang H, Gauthier BR, Hagenfeldt-Johansson KA, Iezzi M, Wollheim CB. Foxa2 (HNF3beta ) controls multiple genes implicated in metabolism-secretion coupling of glucose-induced insulin release. J Biol Chem. 2002 May 17;277(20)

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