Goat anti-ECT2 Antibody |
| Principle Name | ECT2 |
| Official Symbol | ECT2 |
| All Name and Symbols | ECT2; epithelial cell transforming sequence 2 oncogene; FLJ10461; epithelial cell transforming sequence 2 oncogene protein |
| Accession Number(s) | NP_060568.3 |
| Peptide Sequence | AENSVLTSTTGRT, is from N Terminus |
| Human Gene ID(s) | 1894 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 100kDa band reported in human U87-MG glioblastoma lysate at 2.5µg/ml. A 30kDa band of unknown identity was also observed. |
| Reference(s) | Tatsumoto T, Xie X, Blumenthal R, Okamoto I, Miki T. Human ECT2 is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis. J Cell Biol. 1999 Nov 29;147(5) |
