Goat anti-FOXC2 Antibody |
| Principle Name | FOXC2 |
| Official Symbol | FOXC2 |
| All Name and Symbols | FOXC2; forkhead box C2 (MFH-1, mesenchyme forkhead 1); MFH1; MFH-1; FKHL14; MFH-1,mesenchyme forkhead 1; forkhead (Drosophila)-like 14; forkhead, Drosophila, homolog-like 14; forkhead (Drosophila)-like 14 (MFH-1, mesenchyme forkhead 1); LD; forkhead box C |
| Accession Number(s) | NP_005242.1 |
| Peptide Sequence | RHAAPYSYDCTKY, is from C Terminus |
| Human Gene ID(s) | 2303 |
| Non-Human GeneID(s) | 14234 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC, WB trf |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC2 with DYKDDDDK tag probed with EB06007 (1.0ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB06007 (1mg/ml) i |
| Immunohistochemistry | In paraffin embedded Human Bresast cancer shows nuclear staning with some weak cytoplasm staining. Recommended concentration, 2-4µg/ml. |
| Reference(s) | Brice G, Mansour S, Bell R, Collin JR, Child AH, Brady AF, Sarfarazi M, Burnand KG, Jeffery S, Mortimer P, Murday VA. Analysis of the phenotypic abnormalities in lymphoedema-distichiasis syndrome in 74 patients with FOXC2 mutations or linkage to 16q24. J Med Genet. 2002 Jul;39(7) |
