Goat anti-MLH3 Antibody |
| Principle Name | MLH3 |
| Official Symbol | MLH3 |
| All Name and Symbols | MLH3; mutL homolog 3 (E. coli); HNPCC; HNPCC7; MGC138372; S240II117; mutL (E. coli) homolog 3; mismatch repair gene MLH3; DNA mismatch repair protein MLH3; mutL homolog 3 |
| Accession Number(s) | NP_001035197.1; NP_055196.2 |
| Peptide Sequence | RQSLQQSMPPCEPP, is from C Terminus |
| Human Gene ID(s) | 27030 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize isoform 1 (NP_001035197.1) and isoform 2 (NP_055196.2). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | No signal obtained yet but low background observed in HeLa lysate at up to 2µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? |
| Reference(s) | Lipkin SM, Wang V, Jacoby R, Banerjee-Basu S, Baxevanis AD, Lynch HT, Elliott RM, Collins FS. MLH3 |