Goat anti-GNIP / TRIM7 Antibody

Item#:

dAP-0200

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-GNIP / TRIM7 Antibody
Principle Name	GNIP / TRIM7
Official Symbol	TRIM7
All Name and Symbols	TRIM7; GNIP1; RNF90; tripartite motif-containing 7; tripartite motif protein TRIM7; glycogenin-interacting protein 1; GNIP; glycogenin-interacting protein
Accession Number(s)	NP_203128.1
Peptide Sequence	QAPAGPPWDITEA, is from C Terminus
Human Gene ID(s)	81786
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 25-30kDa band observed in Human Skeletal Muscle lysate (predicted MW of 25kDa according to NP_203128). Recommended for use at 1µg/ml.
Immunohistochemistry	In paraffin embedded Human Skeletal Muscle shows pixulate staining in the fibres. Recommended concentration, 3-6µg/ml.
Reference(s)	Skurat AV, Dietrich AD, Zhai L, Roach PJ. GNIP, a novel protein that binds and activates glycogenin, the self-glucosylating initiator of glycogen biosynthesis. J Biol Chem. 2002 May 31;277(22)