Goat anti-CARD6 Antibody |
| Principle Name | CARD6 |
| Official Symbol | CARD6 |
| All Name and Symbols | CARD6; caspase recruitment domain family, member 6; caspase recruitment domain protein 6; CINCIN1; CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation |
| Accession Number(s) | NP_115976.2 |
| Peptide Sequence | ATESTPSEIIERER, is from N Terminus |
| Human Gene ID(s) | 84674 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx. 45-50kDa and 25-30kDa bands observed in A549, Human Heart and Human Skeletal Muscle lysates at 1ug of primary. Please note that the bands we observe are different from the predicted size of approx. 119kDa according to NP_115976. Cur |
| Immunohistochemistry | In paraffin embedded Human Pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Stehlik C, Hayashi H, Pio F, Godzik A, Reed JC. CARD6 is a modulator of NF-kB activation by Nod1- and Cardiak-mediated pathways. J Biol Chem. 2003 May 29 [Epub ahead of print].PMID |
