Goat anti-ALMS1 Antibody |
| Principle Name | ALMS1 |
| Official Symbol | ALMS1 |
| All Name and Symbols | ALMS1; Alstrom syndrome 1; ALSS; KIAA0328; DKFZp686A118; DKFZp686D1828 |
| Accession Number(s) | NP_055935.4 |
| Peptide Sequence | RVTNQLLGRKVPWD, is from C Terminus |
| Human Gene ID(s) | 7840 |
| Non-Human GeneID(s) | 236266 (mouse); 297408 (rat); |
| Important Comments | The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave bands at approx 200kDa and 75kDa in lysates of Human T cell line Jurkat after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given |
| Immunohistochemistry | In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. Recommended concentration |
| Reference(s) | Collin GB, Marshall JD, Ikeda A, So WV, Russell-Eggitt I, Maffei P, Beck S, Boerkoel CF, Sicolo N, Martin M, Nishina PM, Naggert JK. Mutations in ALMS1 cause obesity, type 2 diabetes and neurosensory degeneration in Alstrom syndrome. Nat Genet. 2002 May;31(1) |
