Goat anti-UBC3B Antibody |
| Principle Name | UBC3B |
| Official Symbol | UBE2R2 |
| All Name and Symbols | UBE2R2; UBC3B; CDC34B; FLJ20419; MGC10481; ubiquitin-conjugating enzyme E2R 2; ubiquitin-conjugating enzyme UBC3B |
| Accession Number(s) | NP_060281 |
| Peptide Sequence | AQQQMTSSQKALM, is from N Terminus |
| Human Gene ID(s) | 54926 |
| Non-Human GeneID(s) | 67615 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IEM, IHC |
| Tested Species | Human, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 4000. |
| Western Blot | No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? |
| Immunohistochemistry | In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes . Recommended concentration, 2-4µg/ml. |
| Reference(s) | Semplici F, Meggio F, Pinna LA, Oliviero S. CK2-dependent phosphorylation of the E2 ubiquitin conjugating enzyme UBC3B induces its interaction with beta-TrCP and enhances beta-catenin degradation. Oncogene. 2002 Jun 6;21(25) |
