Goat anti-HSPC150 / UBE2T Antibody

Item#:

dAP-0172

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-HSPC150 / UBE2T Antibody
Principle Name	HSPC150 / UBE2T
Official Symbol	UBE2T
All Name and Symbols	UBE2T; HSPC150; PIG50; HSPC150 protein similar to ubiquitin-conjugating enzyme; ubiquitin conjugating enzyme; ubiquitin-conjugating enzyme E2T (putative); ubiquitin-conjugating enzyme E2T
Accession Number(s)	NP_054895.1
Peptide Sequence	QLVGIEKKFHPDV, is from C Terminus
Human Gene ID(s)	29089
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx. 22kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5kDa according to NP_054895.1). Recommended concentration
Reference(s)	Machida YJ, Machida Y, Chen Y, Gurtan AM, Kupfer GM, D'Andrea AD, Dutta A. UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation. Mol Cell. 2006 Aug;23(4)