Goat anti-GERP / TRIM8 Antibody

Item#:

dAP-0171

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-GERP / TRIM8 Antibody
Principle Name	GERP / TRIM8
Official Symbol	TRIM8
All Name and Symbols	TRIM8; tripartite motif-containing 8; GERP; RNF27; ring finger protein 27; tripartite motif protein TRIM8; glioblastoma expressed ring finger protein; RP11-47A8.4;
Accession Number(s)	NP_112174.2
Peptide Sequence	YGQPSTKHYVTS, is from C Terminus
Human Gene ID(s)	81603
Non-Human GeneID(s)	93679 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	No signal obtained yet but low background observed in human brain and human kidney lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry	In paraffin embedded Human Kidney shows preferential nuclear staining in convoluted tubules. Recommended concentration, 4-6µg/ml.
Reference(s)	Vincent SR, Kwasnicka DA, Fretier P. A novel RING finger-B box-coiled-coil protein, GERP. Biochem Biophys Res Commun. 2000 Dec 20;279(2)