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Goat anti-MURF3 / TRIM54 Antibody
Item#:
dAP-0169
Size Price Quantity
50.0 ug $310.00
Goat anti-MURF3 / TRIM54 Antibody
Principle Name MURF3 / TRIM54
Official Symbol TRIM54
All Name and Symbols TRIM54; MURF-3; tripartite motif-containing 54; RNF30; MURF; ring finger protein 30; muscle-specific RING-finger protein 3; muscle-specific RING-finger protein homolog; OTTHUMP00000122598
Accession Number(s) NP_115935.3; NP_912730.2
Peptide Sequence GSAGPEEERPDGP, is from C Terminus
Human Gene ID(s) 57159
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot Experiments gave bands at approx 45kDa and 38kDa in Human Skeletal Muscle lysate after 1µg/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1kDa according to NP_115935, and 38.6kDa according to
Immunohistochemistry Paraffin embedded Human Skeletal Muscle. Recommended concentration
Reference(s) Centner T, Yano J, Kimura E, McElhinny AS, Pelin K, Witt CC, Bang ML, Trombitas K, Granzier H, Gregorio CC, Sorimachi H, Labeit S. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J Mol Biol. 2001 Mar 2;306(4)

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