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Goat anti-BERP / RNF22 Antibody
Item#:
dAP-0167
Size Price Quantity
50.0 ug $310.00
Goat anti-BERP / RNF22 Antibody
Principle Name BERP / RNF22
Official Symbol TRIM3
All Name and Symbols TRIM3; BERP; RNF22; TRIM3; tripartite motif-containing 3; HAC1; RNF97; ring finger protein 22; brain expressed ring finger; tripartite motif protein TRIM3; FLJ16135
Accession Number(s) NP_006449.2; NP_150594.2
Peptide Sequence AKREDSPGPEVQP, is from N Terminus
Human Gene ID(s) 10612
Non-Human GeneID(s) 55992 (mouse); 83616 (rat);
Important Comments Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 80kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates and approx 85kDa band seen in Mouse Brain (calculated MW of 80.8kDa according to Human NP_006449.2, Mouse NP_001272799.1 and Rat NP_113974.1). Recommended concent
Reference(s) El-Husseini AE, Fretier P, Vincent SR. Cloning and characterization of a gene (RNF22) encoding a novel brain expressed ring finger protein (BERP) that maps to human chromosome 11p15.5. Genomics. 2001 Feb 1;71(3)

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