Goat anti-AXIN1 Antibody |
| Principle Name | AXIN1 |
| Official Symbol | AXIN1 |
| All Name and Symbols | AXIN1; axin 1; AXIN; LA16c-314G4.3; MGC52315; axis inhibition protein 1; axis inhibitor 1; fused, mouse, homolog of |
| Accession Number(s) | NP_003493.1; NP_851393.1 |
| Peptide Sequence | FEEKIIGKVEKVD, is from C Terminus |
| Human Gene ID(s) | 8312 |
| Non-Human GeneID(s) | 12005 (mouse); 79257 (rat); |
| Important Comments | This antibody is expected to recognise both reported isoforms. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Preliminary experiments gave bands at approx 150kDa and 22-24kDa in Human Brain (Hippocampus) lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe give |
| Immunohistochemistry | In paraffin embedded Human Adrenal Gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Zeng L, Fagotto F, Zhang T, Hsu W, Vasicek TJ, Perry WL 3rd, Lee JJ, Tilghman SM, Gumbiner BM, Costantini F. The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation. Cell. 1997 Jul 11;90(1) |
