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Goat anti-Alsin / ALS2 Antibody
Item#:
dAP-0159
Size Price Quantity
50.0 ug $310.00
Goat anti-Alsin / ALS2 Antibody
Principle Name Alsin / ALS2
Official Symbol ALS2
All Name and Symbols ALS2; amyotrophic lateral sclerosis 2 (juvenile); ALSJ; PLSJ; IAHSP; KIAA1563; ALS2CR6; FLJ31851; MGC87187; alsin
Accession Number(s) NP_065970.2
Peptide Sequence LKACYYQIQREKLN, is from C Terminus
Human Gene ID(s) 57679
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot No signal obtained yet but low background observed in Human Brain extracts at upto 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Immunohistochemistry In paraffin embedded Human Cortex shows pixulate cytoplasm staining in some neuronal cells. Recommended concentration, 3-5µg/ml.
Reference(s) Yang Y, Hentati A, Deng HX, Dabbagh O, Sasaki T, Hirano M, Hung WY, Ouahchi K, Yan J, Azim AC, Cole N, Gascon G, Yagmour A, Ben-Hamida M, Pericak-Vance M, Hentati F, Siddique T. The gene encoding alsin, a protein with three guanine-nucleotide exchange factor domains, is mutated in a form of recessive amyotrophic lateral sclerosis. Nat Genet. 2001 Oct;29(2)

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