Goat anti-H11 / HSP22 Antibody

Item#:

dAP-0157

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-H11 / HSP22 Antibody
Principle Name	H11 / HSP22
Official Symbol	HSPB8
All Name and Symbols	HSPB8; heat shock 22kDa protein 8; CMT2L; DHMN2; E2IG1; H11; HMN2; HMN2A; HSP22; Charcot-Marie-Tooth disease, spinal; heat shock 27kDa protein 8; hereditary motor neuropathy, distal; protein kinase H11; small stress protein-like protein HSP22; spinal musc
Accession Number(s)	NP_055180.1
Peptide Sequence	NELPQDSQEVTCT, is from C Terminus
Human Gene ID(s)	26353
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 20-25kDa band seen in Human Skeletal Muscle lysate [Predicted MW of approx. 22kDa according to NP_055180]. Recommended for use at 0.5-1µg/ml.
Reference(s)	Benndorf R, Sun X, Gilmont RR, Biederman KJ, Molloy MP, Goodmurphy CW, Cheng H, Andrews PC, Welsh MJ. HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27). J Biol Chem. 2001 Jul 20;276(29)