Goat anti-NUMB Antibody |
| Principle Name | NUMB |
| Official Symbol | NUMB |
| All Name and Symbols | NUMB; numb homolog (Drosophila); S171; numb homolog |
| Accession Number(s) | NP_001005743.1; 001005744.1; NP_003735; NP_001005745.1 |
| Peptide Sequence | PFSSDLQKTFEIEL, is from C Terminus |
| Human Gene ID(s) | 8650 |
| Non-Human GeneID(s) | 18222 (mouse); |
| Important Comments | This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, ICC, IP |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 80Da band observed in Mouse and Rat Brain lysates (calculated MW of 70.8kDa according to NP_001005743.1). Recommended concentration |
| Reference(s) | Zhong W, Feder JN, Jiang MM, Jan LY, Jan YN. Asymmetric localization of a mammalian numb homolog during mouse cortical neurogenesis. Neuron. 1996 Jul;17(1) |
