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Goat anti-Npap60 / Nup50 Antibody


Item#:
dAP-0142
Size Price Quantity
50.0 ug $310.00
Goat anti-Npap60 / Nup50 Antibody
Principle Name Npap60 / Nup50
Official Symbol NUP50
All Name and Symbols NUP50; NPAP60; NPAP60L; MGC39961; DKFZP564A043; nucleoporin 50kDa; nuclear pore-associated protein 60L; CTA-268H5.7; OTTHUMP00000199530
Accession Number(s) NP_009103.2; NP_705931.1
Peptide Sequence ELHKILLEKKDA, is from C Terminus
Human Gene ID(s) 10762
Non-Human GeneID(s) 18141 (mouse);
Important Comments This antibody is expected to recognise both reported isoforms (NP_009103.2; NP_705931.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 60kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1µg/ml.
Immunohistochemistry In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8µg/ml.
Reference(s) Lindsay ME, Plafker K, Smith AE, Clurman BE, Macara IG. Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha

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