Goat anti-MAD3 / MXD3 Antibody

Item#:

dAP-0140

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MAD3 / MXD3 Antibody
Principle Name	MAD3 / MXD3
Official Symbol	MXD3
All Name and Symbols	MXD3; MAD3; MAX dimerization protein 3; MGC2383; hypothetical protein MGC2383; likely ortholog of mouse Max dimerization protein 3; BHLHC13; FLJ35523; MYX; Max-associated protein 3; Max-interacting transcriptional repressor MAD3
Accession Number(s)	NP_112590.1
Peptide Sequence	QEHSYSHGGGAWL, is from C Terminus
Human Gene ID(s)	83463
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise isoform a (NP_112590.1) only.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IF, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	No signal obtained yet but low background observed in NCI-H460, HepG2, Human Liver, Human Heart and Human Brain lysates at up to 3µg/ml.
Immunohistochemistry	An anonymous customer found positive results in IHC on Human HeLa cells.
Reference(s)	Hurlin PJ, Queva C, Koskinen PJ, Steingrimsson E, Ayer DE, Copeland NG, Jenkins NA, Eisenman RN. Mad3 and Mad4