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Goat anti-UBE2C / UBCH10 Antibody


Item#:
dAP-0136
Size Price Quantity
50.0 ug $310.00
Goat anti-UBE2C / UBCH10 Antibody
Principle Name UBE2C / UBCH10
Official Symbol UBE2C
All Name and Symbols UBE2C; UBCH10; dJ447F3.2; ubiquitin-conjugating enzyme E2C; ubiquitin-protein ligase C; ubiquitin carrier protein E2-C; cyclin-selective ubiquitin carrier protein; mitotic-specific ubiquitin-conjugating enzyme; RP3-447F3.1
Accession Number(s) NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1; NP_001268670.1
Peptide Sequence QETYSKQVTSQEP, is from C Terminus
Human Gene ID(s) 11065
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IF, FC, IP
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1). Recommended concentration
Reference(s) Townsley FM, Aristarkhov A, Beck S, Hershko A, Ruderman JV. Dominant-negative cyclin-selective ubiquitin carrier protein E2-C/UbcH10 blocks cells in metaphase. Proc Natl Acad Sci U S A 1997 Mar 18;94(6)

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