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Goat anti-PI31 / PSMF1 (Isoform 1) Antibody
Item#:
dAP-0130
Size Price Quantity
50.0 ug $310.00
Goat anti-PI31 / PSMF1 (Isoform 1) Antibody
Principle Name PI31 / PSMF1 (Isoform 1)
Official Symbol PSMF1
All Name and Symbols PSMF1; PI31; proteasome (prosome, macropain) inhibitor subunit 1 (PI31); RP4-545L17.1; proteasome inhibitor hP131 subunit; proteasome inhibitor subunit 1
Accession Number(s) NP_006805.2
Peptide Sequence DHLPPPGYDDMYL, is from C Terminus
Human Gene ID(s) 9491
Non-Human GeneID(s) 228769 (mouse);
Important Comments This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 35kDa band observed in Human Kidney lysates (calculated MW of 29.8kDa according to NP_006805.2). In transfected HEK293 transiently expressing PSMF1 a band of approx. 35kDa is observed. This band is not observed in the non-transfected
Reference(s) McCutchen-Maloney SL, Matsuda K, Shimbara N, Binns DD, Tanaka K, Slaughter CA, DeMartino GN. cDNA cloning, expression, and functional characterization of PI31, a proline-rich inhibitor of the proteasome. J Biol Chem. 2000 Jun 16;275(24)

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