Goat anti-SOCS4 Antibody |
| Principle Name | SOCS4 |
| Official Symbol | SOCS4 |
| All Name and Symbols | SOCS4; suppressor of cytokine signaling 4; DKFZp686J1568; SOCS7; SH2 domain containing SOCS box protein; suppressor of cytokine signaling 7 |
| Accession Number(s) | NP_543143.1; NP_955453.1 |
| Peptide Sequence | SKVRVLRIDAPEQQ, is from C Terminus |
| Human Gene ID(s) | 122809 |
| Non-Human GeneID(s) | |
| Important Comments | Both variants represent identical protein (NP_543143.1 and NP_955453.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments in Human and Rodent Brain lysates and in lysates of cell lines NIH3T3, A431 and HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people i |
| Immunohistochemistry | In paraffin embedded Human Pancreas shows cytoplasm staining on select beta cells in islet of Langerhans. Recommended concentration, 3-6µg/ml. |
| Reference(s) | Kario E, Marmor MD, Adamsky K, Citri A, Amit I, Amariglio N, Rechavi G, Yarden
Y. Suppressors of cytokine signaling 4 and 5 regulate epidermal growth factor
receptor signaling. J Biol Chem. 2005 Feb 25;280(8) |
