Goat anti-IRF5 Antibody |
| Principle Name | IRF5 |
| Official Symbol | IRF5 |
| All Name and Symbols | IRF5; interferon regulatory factor 5; SLEB10 |
| Accession Number(s) | NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1 |
| Peptide Sequence | QGPWPMHPAGMQ, is from C Terminus |
| Human Gene ID(s) | 3663 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1). Reported variants NP_001092100.1, NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 60kDa band observed in Human Spleen and Peripheral Blood Lymphocytes lysates.The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration |
| Immunohistochemistry | This antibody showed nuclear staining in Human Cervix, as confirmed by customer (data not shown). This data was obtained using a previous batch. |
| Reference(s) | Barnes BJ, Moore PA, Pitha PM. Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes. J Biol Chem. 2001 Jun 29;276(26) |