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Goat anti-SMARCA3 Antibody
Item#:
dAP-0101
Size Price Quantity
50.0 ug $310.00
Goat anti-SMARCA3 Antibody
Principle Name SMARCA3
Official Symbol HLTF
All Name and Symbols HLTF; SMARCA3; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 3; SNF2-like 3; sucrose nonfermenting-like 3; helicase-like transcription factor; DNA-binding prote
Accession Number(s) NP_003062.2; NP_620636.1
Peptide Sequence KQAKINEIRTLIDL, is from C Terminus
Human Gene ID(s) 6596
Non-Human GeneID(s) 20585 (mouse);
Important Comments NP_003062.2 and NP_620636.2 are varients that represent the same protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 120kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 0.2-1µg/ml. Please note that the weaker smaller band may represent a novel isoform of this protein and is in agreement with previous Western blot observation
Reference(s) Sheridan PL, Schorpp M, Voz ML, Jones KA. Cloning of an SNF2/SWI2-related protein that binds specifically to the SPH motifs of the SV40 enhancer and to the HIV-1 promoter. J Biol Chem. 1995 Mar 3;270(9)

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