Goat anti-KLF8 / BKLF3 Antibody

Item#:

dAP-0100

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-KLF8 / BKLF3 Antibody
Principle Name	KLF8 / BKLF3
Official Symbol	KLF8
All Name and Symbols	KLF8; BKLF3; Kruppel-like factor 8; RP13-1021K9.1; DKFZp686O08126; DXS741; MGC138314; ZNF741; zinc finger protein 741; basic kruppel-like factor 3
Accession Number(s)	NP_009181.2; NP_001152768.1
Peptide Sequence	VDMDKLINNLEVQ, is from N Terminus
Human Gene ID(s)	11279
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise isoform 1 (NP_009181.2) and isoform 2 (NP_001152768.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2) . In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al . J Biol Chem. 2005 Feb 25;280(8)
Immunohistochemistry	Paraffin embedded Human Small Intestine. Recommended concentration
Reference(s)	van Vliet J, Turner J, Crossley M. Human Kruppel-like factor 8