Goat anti-DAP3 Antibody |
| Principle Name | DAP3 |
| Official Symbol | DAP3 |
| All Name and Symbols | DAP3; death associated protein 3; DAP-3; DKFZp686G12159; MGC126058; MGC126059; MRP-S29; MRPS29; bMRP-10; death-associated protein 3; mitochondrial 28S ribosomal protein S29 |
| Accession Number(s) | NP_387506.1; NP_001186779.1; NP_001186780.1 |
| Peptide Sequence | NPSLLERHCAYL, is from C Terminus |
| Human Gene ID(s) | 7818 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC, ICC, IF |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 45.6kDa according to NP_387506.1). The band fades in HeLa after si-RNA-mediated knock-down (including the fainter shoulder band at 37kDa). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Tonsil and Brain (Cortex). Recommended concentration |
| Reference(s) | Kissil JL, Deiss LP, Bayewitch M, Raveh T, Khaspekov G, Kimchi A. Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death. J Biol Chem. 1995 Nov 17;270(46) |
