Goat anti-GRB7 (N Terminus) Antibody

Item#:

dAP-0084

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-GRB7 (N Terminus) Antibody
Principle Name	GRB7 (N Terminus)
Official Symbol	GRB7
All Name and Symbols	GRB7; growth factor receptor-bound protein 7; OTTHUMP00000164352
Accession Number(s)	NP_005301.2
Peptide Sequence	ELDLSPPHLSSSPE, is from N Terminus
Human Gene ID(s)	2886
Non-Human GeneID(s)	14786 (mouse);
Important Comments	This antibody recognises the longer isoform of this protein in A431 lysate.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 60kDa band observed in lysates of cell line A431 (calculated MW of 59.7kDa according to NP_005301.2). In transfected HEK293 transiently expressing GRB7 a band of approx. 60kDa is observed. This band is not observed in the non-transfe
Reference(s)	Margolis B, Silvennoinen O, Comoglio F, Roonprapunt C, Skolnik E, Ullrich A, Schlessinger J. High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains. Proc Natl Acad Sci U S A. 1992 Oct 1;89(19)