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Goat anti-DKK3 / REIC Antibody
Item#:
dAP-0079
Size Price Quantity
50.0 ug $310.00
Goat anti-DKK3 / REIC Antibody
Principle Name DKK3 / REIC
Official Symbol DKK3
All Name and Symbols DKK3; REIC; dickkopf homolog 3 (Xenopus laevis); DKK-3; RIG-like 5-6; RIG-like 7-1; dickkopf 3; Dickkopf gene 3; dickkopf (Xenopus laevis) homolog 3; dickkopf homolog 3; RIG; regulated in glioma
Accession Number(s) NP_037385.2; NP_056965.3; NP_001018067.1
Peptide Sequence AAAALLGGEEI, is from C Terminus
Human Gene ID(s) 27122
Non-Human GeneID(s)
Important Comments NP_037385.2; NP_056965.3 and NP_001018067.1 are varients of the same protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx. 70kDa band observed in lysates of cell line HEK293 (calculated MW of 38.4kDa according to NP_037385.2, NP_056965.3 and NP_001018067.1). The observed molecular weight corresponds to earlier findings in literature (Krupnik et al, Gene
Immunohistochemistry In paraffin embedded Human Brain Cortex shows vesiculate staining in cytoplasm of neuronal cell bodies. Recommended concentration
Reference(s) Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl AD, Gearing DP, Sokol SY, McCarthy SA. Functional and structural diversity of the human Dickkopf gene family. Gene. 1999 Oct 1;238(2)

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