Goat anti-RP11 / PRPF31 Antibody

Item#:

dAP-0076

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-RP11 / PRPF31 Antibody
Principle Name	RP11 / PRPF31
Official Symbol	PRPF31
All Name and Symbols	PRPF31; PRP31 pre-mRNA processing factor 31 homolog (yeast); RP11; PRP31; NY-BR-99; DKFZp566J153; pre-mRNA processing factor 31 homolog (yeast); Protein 61K; PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae); pre-mRNA processing factor 31 homolo
Accession Number(s)	NP_056444.3
Peptide Sequence	LKVKGEKSGLMST, is from C Terminus
Human Gene ID(s)	26121
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 60kDa band observed in lysates of cell line A431 (calculated MW of 55.4kDa according to NP_056444.3. Recommended concentration
Reference(s)	Makarova OV, Makarov EM, Liu S, Vornlocher HP, Luhrmann R. Protein 61K, encoded by a gene (PRPF31) linked to autosomal dominant retinitis pigmentosa, is required for U4/U6*U5 tri-snRNP formation and pre-mRNA splicing. EMBO J. 2002 Mar 1;21(5)