Goat anti-NQO1 Antibody |
| Principle Name | NQO1 |
| Official Symbol | NQO1 |
| All Name and Symbols | NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H |
| Accession Number(s) | NP_000894.1; NP_001020604.1; NP_001020605.1 |
| Peptide Sequence | SIPTDNQIKARK, is from C Terminus |
| Human Gene ID(s) | 1728 |
| Non-Human GeneID(s) | 18104 (mouse); |
| Important Comments | This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IF, IHC |
| Tested Species | Human, Rat, Pig |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 30kDa band observed in Human Kidney and Lung lysates and in Rat and Pig Kidney lysates (calculated MW of 30.9kDa according to NP_000894.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Kidney. Recommended concentration |
| Reference(s) | Asher G, Lotem J, Kama R, Sachs L, Shaul Y. NQO1 stabilizes p53 through a distinct pathway. Proc Natl Acad Sci U S A. 2002 Mar 5;99(5) |
