Goat anti-ELMO2 Antibody |
| Principle Name | ELMO2 |
| Official Symbol | ELMO2 |
| All Name and Symbols | ELMO2; engulfment and cell motility 2 (ced-12 homolog, C. elegans); CED12; FLJ11656; KIAA1834; ced-12 homolog 2; PH domain protein CED12A; engulfment and cell motility 2; engulfment and cell motility 2; RP11-394O2.2; CED-12; ELMO-2 |
| Accession Number(s) | NP_877496.1; NP_573403.1 |
| Peptide Sequence | PKEPSSYDFVYHYG, is from C Terminus |
| Human Gene ID(s) | 63916 |
| Non-Human GeneID(s) | 140579 (mouse); |
| Important Comments | NP_877496.1 and NP_573403.1 are varients that encode the same protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 80kDa band seen in Human Brain lysate. Recommended for use at 1-3µg/ml. |
| Immunohistochemistry | In paraffin embedded Human Prostate shows pixulated staining in the cytoplasm of stroma cells. Recommended concentration |
| Reference(s) | Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, Walk SF, Nemergut ME, Macara IG, Francis R, Schedl T, Qin Y, Van Aelst L, Hengartner MO, Ravichandran KS. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell. 2001 Oct 5;107(1) |
