Goat anti-GRB2 Antibody |
| Principle Name | GRB2 |
| Official Symbol | GRB2 |
| All Name and Symbols | GRB2; growth factor receptor-bound protein 2; ASH; ASH protein; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; HT027; abundant SRC homology; epidermal growth factor receptor-binding protein GRB2; growth factor receptor-bound protein 3; NCKAP2; OTTHUMP00000166096; |
| Accession Number(s) | NP_002077.1; NP_987102.1 |
| Peptide Sequence | PRNYVTPVNRNV, is from C Terminus |
| Human Gene ID(s) | 2885 |
| Non-Human GeneID(s) | 14784 (mouse); 81504 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Mouse, Rat, Pig |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 26kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use |
| Immunohistochemistry | In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration |
| Reference(s) | Lowenstein EJ, Daly RJ, Batzer AG, Li W, Margolis B, Lammers R, Ullrich A, Skolnik EY, Bar-Sagi D, Schlessinger J. The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling. Cell. 1992 Aug 7;70(3) |
