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Goat anti-COP1 / PSEUDO-ICE (Isofom 2) Antibody
Item#:
dAP-0053
Size Price Quantity
50.0 ug $310.00
Goat anti-COP1 / PSEUDO-ICE (Isofom 2) Antibody
Principle Name COP1 / PSEUDO-ICE (Isofom 2)
Official Symbol CARD16
All Name and Symbols CARD16; COP; CARD only protein; PSEUDO-ICE; COP1; caspase-1 dominant-negative inhibitor pseudo-ICE; pseudo interleukin-1beta converting enzyme; caspase recruitment domain family, member 16
Accession Number(s) NP_443121.1
Peptide Sequence ETLGLSAGPIPGN, is from C Terminus
Human Gene ID(s) 114769
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize isoform 2 (NP_443121.1) only. In addition there may be cross-reaction with INCA (GeneID 440068; NP_001007233.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Preliminary experiments gave an approx 28-30kDa band in human colon, pancreas, placenta and tonsil lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe
Reference(s) Druilhe A, Srinivasula SM, Razmara M, Ahmad M, Alnemri ES. Regulation of IL-1beta generation by Pseudo-ICE and ICEBERG, two dominant negative caspase recruitment domain proteins. Cell Death Differ. 2001 Jun;8(6)

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