Goat anti-ATF4 Antibody |
| Principle Name | ATF4 |
| Official Symbol | ATF4 |
| All Name and Symbols | ATF4; activating transcription factor 4 (tax-responsive enhancer element B67); CREB2; TXREB; CREB-2; TAXREB67; OTTHUMP00000199130; activating transcription factor 4; cAMP response element-binding protein 2 |
| Accession Number(s) | NP_001666.2; NP_877962.1 |
| Peptide Sequence | EEVRKARGKKRVP, is from C Terminus |
| Human Gene ID(s) | 468 |
| Non-Human GeneID(s) | 11911 (mouse); 79255 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 37kDa band observed in lysate of K562 cell lines (calculated MW of 38.6kDa according to NP_001666.2 and of NP_877962.1). Recommended concentration |
| Reference(s) | Tsujimoto A, Nyunoya H, Morita T, Sato T, Shimotohno K. Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I. J Virol 1991 Mar;65(3) |
