Goat anti-SMAD4 / MADH4 Antibody

Item#:

dAP-0043

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-SMAD4 / MADH4 Antibody
Principle Name	SMAD4 / MADH4
Official Symbol	SMAD4
All Name and Symbols	MADH4; SMAD4; JIP; DPC4; MAD, mothers against decapentaplegic homolog 4 (Drosophila); Mothers against decapentaplegic, Drosophila, homolog of, 4; MAD (mothers against decapentaplegic, Drosophila) homolog 4; SMAD family member 4; OTTHUMP00000163548; SMAD,
Accession Number(s)	NP_005350.1
Peptide Sequence	HTMPIADPQPLD, is from C Terminus
Human Gene ID(s)	4089
Non-Human GeneID(s)	50554 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IP
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave a band at approx 75kDa in lysates of cell line NIH3T3. Please note that currently we cannot find an explanation in the literature
Reference(s)	Kim SK, Fan Y, Papadimitrakopoulou V, Clayman G, Hittelman WN, Hong WK, Lotan R, Mao L. DPC4, a candidate tumor suppressor gene, is altered infrequently in head and neck squamous cell carcinoma. Cancer Res 1996 Jun 1;56(11)